Quarter-comma Meantone with split sharps

The list of export formats is really impressive. It's interesting to note that Hauptwerk (a pipe organ software emulator) is among the supported targets; some early organs used historical temperaments which sound different compared to equal temperament and some of those had split keys [1][2]. [1] This is an example of meantone organ with split sharps [this video] [2] This is an explanation of Meantone and split keys [link]

Except for voice [0], any fretless stringed instrument [1], any fretted string instrument with separate frets for sharps and flats [2], keyboard instruments that allow expression [3], keyboard instruments with split flat/sharp keys [4], and electronic instruments that allow unorthodox tunings without huge hassle [5]... I'm sure I'm missing some, but other than that you're totally right ;) [0] [link] , [link] [1] [link] [2] [link] [3] [link] [4] [this video] [5] [link]

No, and yes. The difference is obvious even those who don't distinguish pitches much (can you hear the difference in the video linked in the article - [this video] ) because the 'wrong' one played in a scale or a chord sounds really, really horrible; that's why we have all the work done on 'tempered' tuning to reduce the gap so that everything is just slightly off.